Amgen Scholars Program

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Prof. Dr. Michael Sattler

Chair Biomolecular NMR-Spectroscopy, Technische Universität München Research Group Head, Helmholtz Zentrum München


Department of Chemistry, Technische Universität München and Institute of Structural Biology, Helmholtz Zentrum München


Chair Biomolecular NMR-Spectroscopy
Bayerisches NMR Zentrum
Department Chemie, Technische Universität München
Mail: Lichtenbergstrasse 4
Building: Ernst-Otto-Fischer-Straße 2
D-85747 Garching

Institute of Structural Biology
Helmholtz Zentrum München, Geb 43b
Ingolstädter Landstrasse 1
D-85764 Neuherberg


Research Focus

Project description: The molecular mechanism of human peroxisomal protein import

Peroxisomes are membrane-enclosed organelles found in almost all eukaryotic cells. Their vital biosynthetic functions are maintained by PEX proteins which transport necessary enzymes across the membrane into peroxisomes via a yet unknown mechanism. In the Sattler lab, we seek to get insights into the structure and function of the import pore formed via liquid-liquid phase separation in the peroxisomal membrane. Advanced biochemical and biophysical techniques are employed to recombinantly produce the human pore components PEX5, PEX13 and PEX14 and investigate these proteins in model membranes and under phase-separated conditions in vitro using light/fluorescence microscopy. The Amgen scholar will be involved in designing (cloning) and optimizing (purification) different protein constructs followed by screening LLPS conditions using microscopy and biochemical characterization, ie protein-protein interactions, using nuclear magnetic resonance (NMR) spectroscopy, electron microscopy (EM) and other biophysical techniques (ITC, SLS), which will ultimately provide foundation to obtain a structural model of the peroxisomal protein import machinery.

Project supervisor: Sebastian Metzler

Required skills:

· Good understanding in molecular biology (cloning and protein purification) and biology of the system,

· Basic knowledge and understanding of biophysical techniques,

· Good organizational and communication (English) skills,

· Fluent in using Microsoft excel/words/powerpoint for presentation and data analyses.